Method of the Year 2009
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Proteomics
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49+ votes
16- votes
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Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase
- Nadia Taouatas, Madalina M Drugan, Albert J R Heck, Shabaz Mohammed,
- Original article citation: Nat Meth 5," 405 - 407, (2008).
- Categories: Proteomics
- Recommended by: Silvia Surinova on 09/15/2008 12:43PM GMT
- Reasons for recommending:
A novel method for de novo sequencing of peptides and the analysis of post-translational modifications using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). Lys-N-digested peptides can be easily sequenced by following the c-type fragment ions produced during electron transfer dissociation (ETD)-based mass spectrometric analysis.
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