Method of the Year 2009
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Biotechnology
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Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
- Original article citation: Nat Meth 5," 49 - 51, (2008).
- Categories: Biotechnology and Genetics and genomics
- Recommended by: Jianquan Ni on 11/10/2008 08:16PM GMT
- Reasons for recommending:
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs have been inserted into the genome randomly using P-element–mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
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