Method of the Year 2009
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Biotechnology
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7+ votes
6- votes
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PreTect method: Multiplex real-time mRNA detection
- Tor Molden,
- Original article citation: Journal of Virological Methods 142," 204 , (2007).
- Categories: Biotechnology, Cell biology, Others, and Microbiology
- Recommended by: Frank Karlsen on 08/12/2009 10:54AM GMT
- Reasons for recommending:
This method can amplify and detect up to 6 mRNA hotspots selected by microarray or other methods in any routine diagnostic setting. The method has been demonstrated to find cervical pre-cancer directly with very high clinical sensitivity and specificity by amplification and detection of 6 different oncogene markers at the same time. The method has been demonstrated in 18 international per-review articles. The method is in use in 20 countries around the world. The method was already demonstrated to be used for cervical cancer screening in 2003. Since than it has been demonstrated to be functional i more than 200 000 clinical samples. The method is ideal for routine diagnostics of 6 different mRNA from any bacteria, virus, parasite or cell in the same sample. The PreTect method can be used on any fluorescence reader only including adaptable software. The PreTect method has been demonstrated to work in sample that is collected in methanol based media for up to one month in room temperature. The PreTect method is independent of the length of the mRNA and can only amplify mRNA and not double stranded DNA. The PreTect method has bee demonstrated to only amplify mRNA in a mixture of purified RNA and DNA.
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