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• It appears to be a wonderful , quick and cost effective method in the area of enzyme kinectics for studying inhibition.

  • Aug 22, 2009
  • Posted by: Shelly Praveen

• New generation matrix-assisted laser desorption ionization-triple quadrupole mass spectrometer (MALDI-QqQMS), is ideally suited for a variety of enzyme assays and screening protocols. This readout provides comparable speeds (at greater than a sample per second) with superior signal-to-background, better reproducibility and a reagent cost of about 3 cents per well versus 60 cents per well for the fluorescent readout system. Importantly, since the MALDI-QqQMS readout is a direct measurement of the substrate and the product (the essences of an enzyme assay) this readout eliminates false positive and false negative inhibitor hits that can plague fluorescence and chemiluminescent systems that often rely on coupled enzyme assays as the readout. The importance of this work to HTS community is the real potential of opening an entirely new service application to the Drug Discovery research in industrial and academic environment and MALDI-QqQMS platform could change the way the industry does High throughput screening.

  • Sep 03, 2009
  • Posted by: Rakesh Rathore

• Please check our recent paper on MS based High throughput Screening: Rathore R, Corr JJ, Lebre DT, Seibel WL, Greis KD . Extending MALDI-QqQ MS Enzyme Screening Assays to Targets with Small Molecule Substrates. Rapid Commun Mass Spectrom; 2009 Sep;23(20):3293-3300. http://www3.interscience.wiley.com/journal/122597719/abstract?CRETRY=1&SRETRY=0

  • Sep 27, 2009
  • Posted by: Rakesh Rathore

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