Method of the Year 2009
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Biotechnology
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13+ votes
7- votes
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A 3D digital atlas of C. elegans and its application to single-cell analyses
- Original article citation: Nat Meth 6," 667 - 672, (2009).
- Categories: Biotechnology, Genetics and genomics, Systems biology, and Cell biology
- Recommended by: Hanchuan Peng on 09/09/2009 02:45AM GMT
- Reasons for recommending:
Isn't it a cool thing to be able to target individual cells uniquely and unambiguously?
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This paper is a perfect example of how experimental data can be turned into knowledge by computational methods. Especially, the authors are working on microscope images, the kind of data that have reshaped biology. Scientists have invented many amazing ways to take pictures of the world of cells, however, they are also overwhelmed by their scale and complexity. How can we harvest the power of modern imaging techniques? This paper presents a complete pipeline to answer this challenging question and succeeded in worms. There is still lots of work to do in bioimage analysis, but if more and more people follow the framework in this paper, another revolution will come, sooner or later.
This ground breaking work sets the first example of systematically generated digital atlas at single cell level. This is also an excellent example of handling very large amount of data from microscopy images. In few years time, as more efforts are being made, this pipeline will be extended to more complicated systems which will reshape the field.