Method of the Year 2009
In 2007 we chose Next Generation Sequencing.
In 2008 we chose Super-resolution Imaging.
Now it is time for you, our readers, to help us choose the Method of the Year 2009. Just sign in using your free nature.com registration and vote on our Methods to Watch from previous years or a paper that a visitor has recommended.
Alternatively, you can recommend a paper that represents a method you believe came into its own in 2009 and will have a wide-ranging impact on biology. This paper can be any recently published paper from this year or past years published in any journal. Just provide a link to the paper or other online description of the method and vote away!
Want more information or want to comment? Read the editorial or go to our blog methagora and comment.
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PreTect method: Multiplex real-time mRNA detection
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Original article citation: Journal of Virological Methods 142," 204 , (2007). - Categories: Biotechnology, Cell biology, Others, and Microbiology
- Recommended by : Frank Karlsen on 08/12/2009 10:54AM GMT
This method can amplify and detect up to 6 mRNA hotspots selected by microarray or other methods in any routine diagnostic setting. The method has been demonstrated to find cervical pre-cancer directly with very high clinical sensitivity and specificity by amplification and detection of 6 different oncogene markers at the same time. The method has been demonstrated in 18 international per-review articles. The method is in use in 20 countries around the world. The method was already demonstrated to be used for cervical cancer screening in 2003. Since than it has been demonstrated to be functional i more than 200 000 clinical samples. The method is ideal for routine diagnostics of 6 different mRNA from any bacteria, virus, parasite or cell in the same sample. The PreTect method can be used on any fluorescence reader only including adaptable software. The PreTect method has been demonstrated to work in sample that is collected in methanol based media for up to one month in room temperature. The PreTect method is independent of the length of the mRNA and can only amplify mRNA and not double stranded DNA. The PreTect method has bee demonstrated to only amplify mRNA in a mixture of purified RNA and DNA. - Comment on this subject: 0 comments made
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Watching single molecules in cells
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Original article citation: Nat Meth 5," 25 - 25, (2008). - Categories: Cell biology, Imaging, and Others
- Recommended by the Editor: Daniel Evanko on 07/23/2009 09:10PM GMT
In vivo measurement of single molecules is a very challenging area technically. But the enormity of the rewards at stake made us highlight, back in 2007, this area of research as one to watch. The level of progress in 2008 was incremental but developments seem to be gradually improving and there is no question that some landmark papers have been published. Has the progress been sufficient? - Comment on this subject: 1 comment made
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