Method of the Year 2009
In 2007 we chose Next Generation Sequencing.
In 2008 we chose Super-resolution Imaging.
Now it is time for you, our readers, to help us choose the Method of the Year 2009. Just sign in using your free nature.com registration and vote on our Methods to Watch from previous years or a paper that a visitor has recommended.
Alternatively, you can recommend a paper that represents a method you believe came into its own in 2009 and will have a wide-ranging impact on biology. This paper can be any recently published paper from this year or past years published in any journal. Just provide a link to the paper or other online description of the method and vote away!
Want more information or want to comment? Read the editorial or go to our blog methagora and comment.
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Protein biochemistry
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167+ votes
16- votes
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Extensive Analysis of the Cytoplasmic Proteome of Human Erythrocytes Using the Peptide Ligand Library Technology and Advanced Mass Spectrometry
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Original article citation: Molecular & Cellular Proteomics 7," 2254 - 2269, (2008). - Categories: Proteomics and Protein biochemistry
- Recommended by : Roberto Sebastiano on 09/18/2009 10:47AM GMT
This combinatorial peptide ligand library is an extraordinary methodology that will certainly make a big revolution in the field of proteomics! Who would have ever thought that RBCs would contain in the minority cytoplasmic proteome no less than 1578 unique gene product? Great piece of work. - Comment on this subject: 3 comments made
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2+ votes
9- votes
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Universal sample preparation method for proteome analysis
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Original article citation: Nat Meth 6," 359 - 362, (2009). - Categories: Proteomics, Biotechnology, Protein biochemistry, and Systems biology
- Recommended by : Peter Nollert on 08/24/2009 11:54PM GMT
1. the filter-aided sample preparation method impacts the 'forgotten half' of proteomics: membrane proteins & their complexes 2. the method described is *very* simple: SDS & urea reagent and a MWCO filter; no new tools required at all - Comment on this subject: 1 comment made
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2+ votes
4- votes
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Peptide Microarrays
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Original article citation: Journal of Proteome research 7," 3900 - 3910, (2008). - Categories: Proteomics, Biotechnology, Protein biochemistry, and Chemical biology
- Recommended by : Ramars Amanchy on 08/24/2009 09:09PM GMT
1. Peptide microarrays is a new technology with tremendous potential 2. Tremendous impact 3. Multitude of applications 4. Complements tradional kinase motif finding algorithms and techniques. 5. cancer/Kinase profiling - Comment on this subject: 1 comment made
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36+ votes
26- votes
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Development of an Inhibitor Screening Platform via Mass Spectrometry
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Original article citation: Journal of Biomolecular Screening 13," 1007 - 1013, (2008). - Categories: Biotechnology, Protein biochemistry, and Proteomics
- Recommended by : Rakesh Rathore on 08/20/2009 05:19PM GMT
-MALDI-QqQMS readout is a direct measurement of the substrate and the product, this readout eliminates false positive and false negative inhibitor in HTS. -Provides comparable speeds, with superior signal-to-background, better reproducibility and a reagent cost of about 3 cents per well versus 60 cents per well for the fluorescent readout. -Have a real potential of opening an entirely new service application to the Drug Discovery research. - Comment on this subject: 3 comments made
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9+ votes
7- votes
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Top-down mass spectrometry
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Original article citation: Nat Meth 5," 24 - 24, (2008). - Categories: Proteomics, Biotechnology, Protein biochemistry, and Cell biology
- Recommended by the Editor: Allison Doerr on 07/27/2009 01:57PM GMT
Top-down mass spectrometry was selected as a Method to Watch at the end of 2007. This tool is particularly useful for analyzing protein post-translational modifications, but it is not yet a high-throughput method. Have we seen the developments needed to drive its limits? - Comment on this subject: 0 comments made
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3+ votes
5- votes
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Structural analysis of macromolecular assemblies
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Original article citation: Nat Meth 5," 23 - 23, (2008). - Categories: Protein biochemistry, Cell biology, Imaging, and Proteomics
- Recommended by the Editor: Allison Doerr on 07/27/2009 01:56PM GMT
In the 2007 Methods to Watch, Nature Methods highlighted how researchers have begun to integrate proteomics techniques, cryo-electron microscopy, X-ray crystallography, NMR spectroscopy and computational tools to tackle the structure of large macromolecular assemblies like the nuclear pore complex. What do you think of the progress in this area since? - Comment on this subject: 1 comment made
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9+ votes
6- votes
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Membrane protein structures
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Original article citation: Nat Meth 6," 35 - 35, (2009). - Categories: Protein biochemistry, Biotechnology, and Cell biology
- Recommended by the Editor: Allison Doerr on 07/27/2009 01:49PM GMT
In 2008, we selected membrane protein structure determination as a Method to Watch. Membrane protein structures are still highly underrepresented in the Protein Data Bank, but thanks to increased efforts to improve expression and crystallization approaches, their numbers are growing. But is it still too soon to consider these efforts for Method of the Year? - Comment on this subject: 0 comments made
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11+ votes
7- votes
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Quantitative mass spectrometry
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Original article citation: Nat Meth 6," 34 - 34, (2009). - Categories: Proteomics, Biotechnology, Protein biochemistry, and Cell biology
- Recommended by the Editor: Allison Doerr on 07/27/2009 01:46PM GMT
Quantitative mass spectrometry-based proteomics has been increasingly applied on a large scale to address interesting biological questions; we thus selected it as a Method to Watch in 2008. Has the technology matured enough yet to be considered as Method of the Year in 2009, or are further methodological developments still needed? - Comment on this subject: 1 comment made
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